H Thomas Karnes, Ph.D.
Emeritus Professor
Department of Pharmaceutics
  •   Smith Building, Room 309
  • School of Pharmacy - Dept of Pharmaceutics
    410 N 12th Street
    Box 980533
    Richmond, VA 23298-0533
  •  (804) 828-3819
    (804) 828-8359


  • Ph.D., Pharmaceutical Sciences (University of Florida, 1984)
  • M.S., Pathology (University of Florida, 1980)
  • B.S., Chemistry (Illinois State University, 1977)
  • A.A.S, Medical Technology (Illinois Central College, 1974)

Professional Experience

  • (2005 - Present) Fellow, VCU Center for the Study of Biological Complexity
  • (2004 - Present) Affiliate Professor, Department of Chemistry, Virginia Commonwealth University
  • (1998 - Present) Affiliate Professor, Department of Medicinal Chemistry, Virginia Commonwealth University
  • (1997 - 2000) Associate Dean for Research and Graduate Studies, School of Pharmacy, Virginia Commonwealth University
  • (1996 - Present) Professor, Department of Pharmaceutics, School of Pharmacy, Virginia Commonwealth University
  • (1995 - Present) Director of Analytical Core Laboratories, Center for Drug Studies, School of Pharmacy, Virginia Commonwealth University
  • (1992 - Present) Affiliate Professor, Biomedical Engineering, Virginia Commonwealth University
  • (1990 - 1995) Director of Analytical laboratories, BioClin, Inc.
  • (1984 - 1990) Director of Analytical Laboratories, Biopharm Center for Clinical Research, Virginia Commonwealth University
  • (1984 - 1989) Assistant Professor, Department of Pharmacy and Pharmaceutics, School of Pharmacy, Virginia Commonwealth University
  • (1981 - 1982) Research Chemist, Department of Pharmacy Practice, University of Florida, Gainesville, FL
  • (1980 - 1981) Analytical Chemist, Analytical Toxicology Laboratory, Shands Teaching Hospital, Gainesville, FL

Professional and Scholarly Interests

  • Bioanalysis using novel luminescence
  • Mass spectroscopy, immunoreactor and laser spectroscopy systems
  • Drug metabolism, drug release and biological drug distribution

Research Interests

  • The research of our group involves the analytical chemistry of biological systems. We believe we are one of the most active groups of this kind in the World. Our work includes investigation of novel instrumental and chemical approaches to provide improved selectivity and sensitivity for quantitative analysis of drugs in biological samples. In past research, we have employed separation approaches such as capillary electrophoresis, and capillary electrochromatography; validation and control approaches for analytical methods; laser induced fluorescence, chemical derivatization to improve detection; chemiluminescence detection in liquid chromatography; on-line immunoreaction detectors and far-red solution spectroscopy. The research laboratory works in conjunction with a well equipped, state of the art bioanalytical core facility that provides students with "real world" experience and a steady source of research funding.


PubMed Search
The publications search is performed on the National Library of Medicine's PubMed database. The search may be slow during high traffic hours, may return articles authored by another researcher with a similar name and may not always find all of a researcher's articles.

Books/Book Chapters

  • Karnes HT: “Validation and Control of Bioanalytical Methods,” In: Figg WD, McLeod H, editors. Handbook of Anti-Cancer Pharmacokinetics and Pharmacodynamics–Cancer Drug Discovery and Development Series, New Jersey: Humana Press; 91-111, 2004.

Recent Publications

  • Shah KA and Karnes HT: “A Review of the Analysis of Tobacco Specific Nitrosamines in Biological Matrices”, Critical Reviews in Toxicology, 40(4): 305-327, 2010.
  • McKinney DL, Davies BD, Gogova M, Ramakrishnan V, Fisher K, Carter H, Karnes HT, Garnett WR, Iyer SS, Somani AA, Kobal G and Barr WH: “Rapid Automated Blood Sampling System for Pharmacokinetic Studies of Cigarette Smoking”, Nicotine and Tobacco Research, 12(4): 319-325, 2010.
  • Shah KA and Karnes HT: “A Proposed "Fixed" Range Decision Criteria for Transfer of Bioanalytical Methods”, Journal of Chromatography B, 877: 2270-2274, 2009.
  • Shah KA, Halquist MS and Karnes HT: “A Modified Method for Determination of Tobacco Specific Nitrosamine 4-(Methyonitrosoamino)-1-(3-pyridyl)-1 Butanol in Urine by Solid Phase Extraction Using Molecularly Imprinted Polymer and Liquid Chromatography Tandem Mass Spectrometry”, J Chromatography B, 877(14-15): 1575-1582, 2009.