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Adam M Hawkridge, Ph.D.
Director, VCU Massey Comprehensive Cancer Center Proteomics Shared Resource Center
Associate Professor
Department of Pharmaceutics / Department of Pharmacotherapy & Outcomes Science
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  •   Smith Building, Room 644
  • School of Pharmacy - Dept of Pharmaceutics
    410 N 12th Street
     Box 980533
     Richmond, VA 23298-0533
  •  (804) 828-1258
    (804) 828-8359
  •  amhawkridge@vcu.edu

Education

  • Ph.D., Chemistry (University at Buffalo, 2000)
  • B.S., Chemistry (Virginia Tech, 1996)

Post-Graduate Training

  • Post Doctoral Fellow - Mayo Clinic (Mayo Proteomics Research Center, 2003-2005)
  • Post Doctoral Fellow - University of Arizona (Department of Chemistry & Biochemistry 2000-2003)

Professional Experience

  • (2014 - Present) Affiliate Member, Massey Comprehensive Cancer Center, Virginia Commonwealth University,
  • (2012 - Present) Assistant Professor, Departments of Pharmaceutics & Pharmacotherapy and Outcomes Science, Virginia Commonwealth University
  • (2005 - 2012) Research Assistant/Associate Professor, W.M. Keck FTMS Laboratory, North Carolina State University

Research Interests

  • Our laboratory focuses on the development of innovative approaches in quantitative mass spectrometry-based proteomics which we in turn use to investigate the onset, progression, diagnosis, and treatment of cancer with unprecedented molecular-level detail. A main area of interest in the group is identifying predictive biomarkers for early-stage diagnosis of cancer which we pursue using a combination of high-performance mass spectrometry, experimental models, and clinical biospecimens. We are currently studying the diagnostic and biological significance of several novel candidate markers in human ovarian cancer cells, plasma, and tumors. A second area of research focuses on identifying molecular signatures that can stratify lung cancer patients for the development of personalized therapeutic strategies. In vitro metabolic labeling (e.g., stable isotope labeling by amino acids in cell culture, SILAC; and bioorthogonal reagents) of lung cancer cell lines combined with quantitative proteomics analysis are being used to identify candidate biomarkers and discover new signaling pathways before, during, and after chemo/radiation treatment. Finally, an overarching effort in the group is to further expand the use of SILAC as a quantitative approach toward clinical specimens. The laboratory is highly collaborative and members are expected to develop strong analytical skills applied to solving important biomedical problems. The laboratory is well equipped with a high-performance instrumentation and cell culture facilities.

Publications

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Recent Publications

  • G. L. Andrews, R. A. Dean, A. M. Hawkridge, D. C. Muddiman, “Maximizing Proteome Coverage of Yeast on an LTQ-Orbitrap Using Design of Experiments”, Journal of the American Society for Mass Spectrometry, 2011, 22 (4), 773-783.
  • A. M. Hawkridge, R. B. Wysocky, J. N. Petitte, K. E. Anderson, P. E. Mozdziak, O.J. Fletcher, J. M. Horowitz, D. C. Muddiman, “Measuring the Plasma Proteome Intra-individual Variability in the Chicken Model of Spontaneous Ovarian Adenocarcinoma”, Analytical and Bioanalytical Chemistry, 2010, 398 (2), 737-749.
  • A. M. Hawkridge and D. C. Muddiman, “Mass Spectrometry-Based Biomarker Discovery: Towards a Global Proteome Index of Individuality”, Annual Review of Analytical Chemistry, 2009, 2, 265-278.
  • A. M. Hawkridge, D. M. Heublein, A. Catialotti, J. C. Burnett, Jr., D. C. Muddiman, “Effect of Plasma Protein Depletion on BNP-32 Recovery” Clinical Chemistry, 2008, 54, 933-934.
  • J. L. Frahm, I. D. Bori, D. L. Comins, A. M. Hawkridge, D. C. Muddiman, “Achieving Augmented Limits of Detection for Peptides with Hydrophobic Alkyl Tags” Analytical Chemistry, 2007, 79(11), 3989-3995.
  • J. Sampson, A. M. Hawkridge, D. C. Muddiman, “Generation and Detection of Multiply-Charged Peptides and Proteins by Matrix-Assisted Laser Desorption Electrospray Ionization (MALDESI) Fourier Transform Ion Cyclotron Resonance Mass Spectrometry” Journal of the American Society for Mass Spectrometry, 2006, 17(12), 1712-1716.
  • A. M. Hawkridge, D. M. Heublein, H. R. Bergen, III, A. Cataliotti, J. C. Burnett, Jr., D. C. Muddiman, “Quantitative Mass Spectral Evidence for the Absence of Circulating Brain Natriuretic Peptide (BNP-32) in Severe Human Heart Failure” Proceedings of the National Academy of Sciences USA, 2005, 102, 17442-17447.